Penicillin thesis

After a sample is obtained, it should be examined by standard histologic methods, anaerobic culture for 2 weeks, and immunofluorescence if available. Culture is the least reliable method of verifying infection (in Fiorino's series only 35% of cultures were positive for fermentative actinomycetes ( r21 ) when standard aerobic and anaerobic culture techniques are used. However, an 86% success rate has been reported for samples cultured in the presence of metronidazole, which inhibits the growth of faster growing anaerobes ( 92 ), and, as indicated by the overall number of culture-proven cases ( 47 , 69 , 81 ) and the percentage of culture-positive IUD specimens ( 17 , 18 ), even better results may be obtained when transparent agar media, Fortner's ( 22 ) method for producing a semianaerobic atmosphere, and microscopic examination of the cultures for up to 14 days of incubation at 36 ± 1°C are used ( 81 ). The last procedure also facilitates the detection of actinomycete colonies among a usually large amount of various colony types of concomitant microbes.

Penicillin thesis

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